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Custom Cell Culture

Cell Lines CAR-T Cells Primary Cells Custom Reporter Cell Lines (e.g. GFP, RFP, Luciferase, among others) Custom shRNA Knockdown Constructs

Based primarily out of North America, under USDA regulated facilities, our partnering labs enjoy a long standing and excellent history in cell production. Our range extends across both traditional Cell Production to unique and novel Cell lines.

  • Cell Lines - Our partner labs will isolate cells of any description from specific organs or tissues (any animal or human tissues) designated. For example, they are well versed in isolating endothelial cells and epithelial cells from animals (wild-type and knock-out murine strains, rat strains) from any desired tissue. The lab will isolate the desired cell type and supply a vial containing the prepared cells ready to use. Any specific requests can be attended to. Email us for more information at Info@2BScientific.com
  • CAR-T Cells - Custom Immune Cell Engineering Services Immunotherapy has become the fourth pillar of cancer therapy, independent of surgery, radio- and chemo-therapy. Recently emerging Chimeric Antigen Receptor (CAR) T cell therapy has shown great promise for cancer treatment. Rockland Inc. is making available its expertise for production of CAR-modified T and other immune cells using both viral and non-viral transfection methodologies. Email us for more information at Info@2BScientific.com
  • Primary Cells - our partner labs are able to isolate cells of any description from specific organs or tissues (any animal or human tissues) designated. For example, they are well versed in isolating endothelial cells and epithelial cells from animals (wild-type and knock-out murine strains, rat strains) from any desired tissue. Whether they are provided either the already excised tissues or live mice or rats, the lab will isolate the desired cell type and return a vial containing the prepared cells ready to use. For further further and specific requirements please email us for more information at Info@2BScientific.com.
  • Custom Reporter Cell Lines (e.g. GFP, RFP, Luciferase, among others) - transcription factor-dependent reporter cell lines can be ideal tools for high-throughput RNAi, peptide, and chemical library screens. Responsive reporter cells form the core of cell-based assays for screening and identifying drugs, antibodies, cell factors, and other effectors. With lentiviral-based transduction, our partner labs can easily engineer stable reporter lines for almost any application–regardless of cell type. To find out more details please email us for more information at Info@2BScientific.com.
  • Custom shRNA Knockdown Constructs - Our partner labs provide complete services for construction of custom constructs expressing shRNA targeting any transcript:
    • Choose constitutive or inducible version of H1 or U6 shRNA promoters
    • Select GFP, RFP, PuroR, BleoR, NeoR, or Hygro-HK markers
    • Obtain constructs as plasmid or packaged lentiviral particles

To make an shRNA construct, the lab only need to know the RefSeq number. They can design any number of shRNA constructs that target the transcript. Thier proprietary shRNA design delivers approximately 70% shRNAs that generate >70% transcript knockdown in standard cell lines as measured by qRT-PCR. To get more details please email us for more information at Info@2BScientific.com

Medium and Feed Development

Our supply partner Xell offers targeted, rapid and efficient services for the development of custom cell culture media and custom feed supplements

They have previously developed custom cell culture media and feeds for cell lines like CHO, BHK, hybridoma, HEK and other human cell lines throughout the biotech industry. All of their media and feed products are chemically defined, animal component-free and available as liquid or powder formulations.

 

Featured Custom Engineered Cell Lines

Try Cellecta custom services to make cell lines with targeted gene knockouts or containing specific knock-in sequences and speed up your time to results.

The approach varies depending on what cell engineering is required:

  • Knock out, knock down or knock in a gene in your choice of cell line
  • Zero-footprint CRISPR knockout using non-integrated sgRNA and Cas9
  • Knock-in genes or gene-tags
  • Inducible gene activation and inhibition with CRISPRa and CRISPRi
  • Reporter cells and other non-CRISPR mediated engineering available


We can provide validated cell pools or clonal cell lines:

  • Cell pools - we sequence and confirm modifications at the target location
  • Clonal lines - we characterize the specific indels or insertions present in each allele
  • Knockout cells - we check for the presence of the wild-type allele since many common cell lines are polyploid