AteloGene Local Use Quick Gelation Citation Available Product Size(s) 1 kit £1273.00 KOU-1492 Add to order Added to order Academic Pricing Please contact us for academic pricing. Contact Us About this Product SKU: KOU-1492 Extra Details: Agreement for purchasing AteloGene: Please fill out the agreement form (PDF file located in Documents tab), then fax or e-mail the completed form to Cosmo Bio USA prior to placing your order with us. See also: AteloGene for Systemic Use Atelogene for Local Use (standard) This product is a kit for delivering siRNA into live mice. Quick gelation at injection sites!AteloGene Local Use "Quick Gelation" (AteloGene QG) and AteloGene Local Use were mixed with siRNA respectively, and were observed for gelation after administration in mouse subcutaneous tissue. Compared with AteloGene Local Use, results for AteloGene QG showed a rapider gelation after administration.Efficient Delivery!AteloGene QG and AteloGene Local Use were mixed with Luciferase siRNA (Luc siRNA) respectively, and evaluated for the luminescence of Luciferase after administration in a Dual-luciferase expressed subcutaneous tumor model. Result with AteloGene QG showed higher inhibition of Luciferase gene expression than results compared to AteloGene Local Use.Increased Volume! Contents of kitThis kit is intended for 15 times of administration.Devices and reagents required other than the kitStorage Storage temperature: 2-10°C (do not freeze) Precautions for storagePrecautions for useMethods"AteloGene® Local Use Quick Gelation" Preparation Instruction In this section, the number 1)-4) refer to the "Kit contents" of the product page. Implement measures to secure an RNase-free environment, as for as possible, to avoid the degradation of siRNA/miRNA in advance.1)Preparation of "AteloGene® Local Use Quick Gelation"Attach the 4) 18G needle to the 1) Prefilled syringe. Eject the whole amount (540 uL) into the 3) 2 mL microtube. After ejection, cool the microtube containing "AteloGene® QG" on ice.Note) An excess amount of "AteloGene® QG" is provided in the 1) Prefilled syringe so that 540 uL of "AteloGene® QG" can be injected into the 3) 2 mL microtube regardless of losses during the preparation steps.2)Preparation of siRNA solutionPrepare 25-50 uM siRNA/miRNA solutions with RNase-free water.3)Preparation of "AteloGene®" & siRNA mixed solution.While cooling on ice, gently pour 540 uL of the 2) QG buffer and then 120 uL of the siRNA/miRNA solution onto 540 uL of " AteloGene® QG" in the 3)2mL microtube. Rotate and mix the solutions at 4°C for 10 minutes. To avoid forming bubbles, the rotation speed should be approximately 12 r.p.m. (in the case of a rotator with a diameter of 20 cm).Note) Although the mixture may become cloudy during this procedure, it will become a clear solution as the components are thoroughly mixed by rotation.4) Deformation of bubbles and preparation for administrationCentrifuge the mixture at 10,000 r.p.m. for 1 minute at 4°C to deform bubbles. Attach the 4)18G needle to the disposable syringe and slowly draw the mixture while avoiding forming bubbles. Replace the needle with the 27G needle and keep the syringe refrigerated until immediatelly before administration., KOU Shipping Conditions: Blue Ice Storage Conditions: 2-8[o]C Supplier: Cosmo Bio Ltd Type: Molecular Biology Reagents: Transfection Size: 1 kit Need Help? View product citations for biochemicals KOU-1492 on CiteAb