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AEC Substrate Kit, Peroxidase (HRP), (3-amino-9-ethylcarbazole)

Product Size(s)
1 Kit
£215.00
SK-4200-1
Academic Pricing
Please contact us for academic pricing.
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About this Product
SKU:
SK-4200
Additional Names:
Enzyme Substrates [Enzyme Substrates ] Precipitating HRP Substrate, Horseradish Peroxidase substrate
Application:
Immunohistochemistry, IHC-Frozen, IHC-Paraffin, Immunocytochemistry, ELISPOT, FLUOROSPOT
Extra Details:
AEC (3-amino-9-ethylcarbazole) and ImmPACT AEC HRP substrates both produce red reaction products that can be used as a single label or as a second color for multiple antigen labeling. AEC and ImmPACT AEC chromogens can be used for both manual and automated staining methods. Stained slides must be aqueously mounted because these reaction products are soluble in organic solvents. Sections stained with AEC are stable for at least 4 years when mounted in VectaMount AQ (H-5501).

This kit contains stock solutions in convenient dropper bottles. This product can also be used on blots. The kit provides all of the necessary reagents to prepare about 300 ml of working solution. When stored at 4 C, this kit is stable for one year.

Features:

  • Greater sensitivity than conventional substrates
  • Consistent and reliable
  • Suitable for IHC/ICC and blots
  • Aqueous mounting
  • Suitable for single and multiple labeling
  • Stock solutions supplied in convenient dropper bottles promoting ease of handling
  • No wait times for mixing and dissolving powders or tablets
  • One year expiry date
  • Sufficient reagents to produce 300 ml of working solution
    • Shipping Conditions:
    Ambient
    Storage Conditions:
    2-8 [o]C
    Supplier:
    Vector Laboratories
    Size:
    1 Kit
    What is the difference between the three different AEC substrate kits that are offered by Vector Laboratories?:
    We have offered conventional AEC peroxidase substrate, Cat. No. SK-4200, for many years. While this is our most economical kit format generating 300 ml of working solution, it is also the least sensitive of the three kits. Use of SK-4200 would be for the detection of abundantly expressed antigens and where assay costs are a main consideration. ImmPACT® AEC, Cat. No. SK-4205, was subsequently introduced and offers several advantages over the conventional SK-4200. In particular, SK-4205 is about 3-5 times greater in sensitivity over SK-4200, and the working solution is stable for two weeks when stored at 4°C. ImmPACT® AMEC Red, Cat. No. SK-4285, is our most sensitive AEC substrate that generates a crisper, brighter red reaction product, and is 5-10 times more sensitive when compared with conventional AEC substrates such as SK-4200. Like ImmPACT® AEC, the ImmPACT® AMEC Red working solution can be stored for two weeks in the fridge without loss of staining performance. We have also determined that where archiving of stained sections is important, ImmPACT® AMEC Red remains localized and undiminished in intensity for at least 2 years when coverslipped with VectaMount® AQ Mounting Medium.
    SK-4200_AEC_HRP_Substrate.jpg

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